Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Dec 12;23(4):372–380. doi: 10.5607/en.2014.23.4.372

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © Experimental Neurobiology 2014.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Fig. 3 — Immunofluorescence analysis of GRP78 and p-eIF2α in CFA-injected rat TG. On the fifth day after CFA-injection in rat vibrissal pad, we examined the expression of ER stress marker proteins in rat TG. (A) In control rats, GRP78 and p-eIF2α were mostly expressed in TG neurons, as demonstrated by colocalization of GRP78 or p-eIF2α (red) and neuronal marker MAP2 (green). (B-C) GRP78 or p-eIF2α immunoreactivity increased remarkably in CFA-injected TG compared to the control TG. Quantification of the GRP78 or p-eIF2α immunopositive intensity increased in CFA-injected TG compared to control TG (right). (D) A significant increase in the number of p-eIF2α immunopositive neurons was observed in the CFA-injected TG. (E) The expression of p-eIF2α increased significantly in small and medium sized neurons after CFA-induced peripheral inflammation. Data is expressed as means±SD of three independent experiments. Scale bar=50 µm. ^*p<0.05 (Student's t-test).