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. 2014 Nov 13;289(52):35668–35684. doi: 10.1074/jbc.M114.618819

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — G_s-biased pepducins do not promote β₂AR internalization or desensitization. A, β₂AR internalization was monitored by cell surface ELISA in HEK 293 cells stably overexpressing FLAG-β₂AR post-stimulation with 1 μm isoproterenol, 5 μm salbutamol, or 10 μm pepducin in DMEM with 10% FBS. 0.05% DMSO was included in non-pepducin-stimulated cells. The data are represented by the mean of three independent experiments ± S.D. B, total cAMP was measured post-stimulation with 1 μm isoproterenol, 5 μm salbutamol, or 10 μm pepducin in DPBS at the indicated time points in human ASM cells. C, intracellular cAMP was measured post-stimulation with 1 μm isoproterenol, 5 μm salbutamol, or 10 μm pepducin in DPBS at the indicated time points in human ASM cells. 0.05% DMSO was included in non-pepducin-stimulated cells. The data are represented by the mean of three independent experiments ± S.D.