FIGURE 6.

Src family kinase inhibition blocks Podoplanin cluster dynamics. A, TIRFM images of Lifeact-GFP-expressing platelets interacting with planar lipid bilayer containing Dylight 594-labeled mPDPN-Fc for 45 min at 37 °C. Lifeact-GFP-expressing platelets were pretreated with Src family kinase inhibitor (PP2, 20 μm) or a Syk inhibitor (PRT-060318, 5 μm). Scale bar, 10 μm. B, quantification of the cluster morphology in the presence and absence of Src family (PP2, 20 μm) or a Syk (PRT-060318, 5 μm) kinase inhibitors at 45 min. Platelets were identified as having a single or multiple Podoplanin clusters. Images and results are the averages of three independent experiments ± S.D.