FIGURE 5.

Autophagy is induced in vivo following GBS infection. CD1 male mice were injected intravenously with WT (n = 9) or ΔcylE GBS strain (n = 9) or injected with PBS (n = 2). A, bacterial counts (cfu) in mouse brain and in blood at the time of death. B, Kaplan-Meier survival plot. Significance was assessed using a log rank (Mantel-Cox) test. C, Western blot analysis was performed on protein lysates from brain harvested from mice infected with NCTC 10/84 and isogenic ΔcylE mutant for LC3-I, LC3-II, and p62. D, image analysis was performed using ImageJ software to determine LC3-II/LC3-I ratios. **, p < 0.005; ****, p < 0.0001. Error bars represent S.D.