FIGURE 2.

LPA did not induce LPA₂ internalization within 5 min of exposure. A, schematic representation of EGFP-tagged LPA₂. B, EGFP-LPA₂ functioned similar as WT-LPA₂ in LPA-induced intracellular Ca²⁺ mobilization. C, TIRF imaging showing the distribution of EGFP-LPA₂ in 3T3-LPA₂ cells before and after treatment with an LPA gradient for 5 min. Scale bar, 10 μm. D, SPT images of FLAG-LPA₂ expressed in HEK293 cells in the absence or presence of LPA. Scale bar, 10 μm. E, quantitative analysis of the percentage of LPA₂ internalization for 30 min with or without LPA treatment (mean ± S.E. (error bars), n = 4). *, significant differences compared with 0 min in each group (p < 0.05).