FIGURE 2.

Core does not increase TG lipogenesis. A, mice injected with adenovirus expressing core and GFP or GFP alone were given an intraperitoneal injection of ²H-labeled saline 4 days after infection. After 5 h, livers samples were harvested and subjected to MS. Deuterium content in TG-palmitate, TG-glycerol, and cholesterol was measured (n = 6 mice/group). B, 24 h after transfection with FLAG-core, DGAT2-FLAG, or LacZ, Cos-7 cells were loaded with radiolabeled [¹⁴C]glucose. 20 h after loading, medium was removed, and lipids were extracted and subjected to TLC. The bands corresponding to TG were excised, and TG radiolabeling (cpm) was determined by liquid scintillation counting, normalized to total protein. Results are representative of two independent experiments with three replicates in each experiment. Data are presented as the mean ± S.D.