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. 2014 Nov 3;289(52):35891–35906. doi: 10.1074/jbc.M114.599597

FIGURE 5.

FIGURE 5.

Delayed germination of B. bassiana on the skins of larvae undergoing ecdysis. B. bassiana conidia were applied to the surfaces of larvae undergoing ecdysis or on feeding stages and tissue were sectioned. A–E, surface application of B. bassiana to larvae undergoing the 4th larval-larval ecdysis. Conidia germination was delayed until 24 h postapplication, but systematic melanization occurred quickly. F–J, application of B. bassiana to feeding larvae (V-2). Conidia germination was observed between 6 and 12 h. Melanization occurred after hyphae passed through the cuticle. K–O, surface application of B. bassiana to larvae undergoing larval-pupal ecdysis. Conidia germination was delayed until 48 h. In A and K, insects at different molting stages became melanized after B. bassiana application (a), and the same insects are shown after the old cuticles were manually removed (b). The arrows indicate the conidia (C, G, and M) and newly germinated hyphae (D, H, and O) on the surfaces of larvae. The arrowheads indicate hyphae inside the cuticle (E, I, and J). OC, old cuticle of ecdysis larvae; NC, new cuticle of ecdysis larvae; C, cuticle of feeding larvae; E, epidermal cells. Scale bar, 10 μm.