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. 2014 Sep 25;289(52):35987–36000. doi: 10.1074/jbc.M114.589119

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — DUE-B interacts with the MCM complex. A, DUE-B^Sf9 was incubated with interphase egg extract and fractionated by gel filtration. Mcm7 and DUE-B were detected using specific antibodies. B, interphase egg extract was treated with RNase A and then incubated with DUE-B^Sf9. C, DUE-B^Sf9 was incubated with benzonase nuclease-treated interphase egg extract and then immunoprecipitated (IP) by affinity purified DUE-B antibody or normal rabbit IgG. The immunoprecipitates were resolved by SDS-PAGE and probed for specific proteins. D, DUE-B^Sf9 was incubated with benzonase nuclease-treated interphase egg extract. Mcm3 or Mcm5 were immunoprecipitated by specific antibodies. The immunoprecipitates were resolved by SDS-PAGE and Western blotted for DUE-B.