FIGURE 3.

PlGF attenuates expression of miR-199a2 and its host gene DNM3os in endothelial cells. A, summary of premirs and miRNA sequences originating from DNM1os, DNM2os, and DNM3os loci, respectively. B and C, HMEC cells were treated with PlGF for 2 h, and expression levels of the indicated premirs were determined in extracted RNA by qRT-PCR. D, HMEC cells were exposed to hypoxia (1% O₂) for 24 h, followed by extraction of RNA for qRT-PCR analysis of indicated premirs and DNM3os. The data represent means ± S.E. for n = 3. ns, not significant.