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. 2014 Nov 4;289(52):36101–36115. doi: 10.1074/jbc.M114.598383

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Ascl2 knockdown in LS174T cells increased miR-200 family expression and reduced ZEB1 and ZEB2 levels in both shRNA-Ascl2/HT-29 cells and shRNA-Ascl2/LS174T cells. The quantitative real time PCR experiments demonstrated an increase in the expression of miR-200 family members in shRNA-Ascl2/LS174T cells compared with shRNA-Ctr/LS174T cells (**, p < 0.01) (A). The protein levels of ZEB1 and ZEB2 in both shRNA-Ascl2/HT-29 cells (B) and shRNA-Ascl2/LS174T cells (E) were reduced compared with control cells; ZEB1 and ZEB2 proteins in tumor xenografts developed from shRNA-Ascl2/HT-29 cells (C) and shRNA-Ascl2/LS174T cells (F) were reduced compared with the control. The mRNA levels of ZEB2 in both shRNA-Ascl2/HT-29 cells and shRNA-Ascl2/LS174T cells were significantly reduced compared with control cells (p < 0.01), and the mRNA level of ZEB1 in shRNA-Ascl2/HT-29 cells was significantly reduced compared with control cells (p < 0.01) (D and G). Error bars represent S.D.