Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Nov 4;289(52):36101–36115. doi: 10.1074/jbc.M114.598383

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 7. — Transient transfection of shRNA-Ascl2/LS174T cells with the miR-200a or miR-429 inhibitor increased the proliferation and migration of shRNA-Ascl2/LS174T cells. Transient transfection of the miR-200a inhibitor or the miR-429 inhibitor into shRNA-Ascl2/LS174T cells significantly increased proliferation between 48 and 96 h post-transfection compared with untreated shRNA-Ascl2/LS174T cells or negative control (NC) inhibitor-transfected shRNA-Ascl2/LS174T cells. Transfection of the miR-200b inhibitor into shRNA-Ascl2/LS174T cells did not have any effect on proliferation (A). The number of cells migrating across the scraped edge under magnification (200×) in shRNA-Ascl2/LS174T cultures transfected with the miR-200a inhibitor or the miR-429 inhibitor was significantly higher than that observed for untransfected shRNA-Ascl2/LS174T cells and negative control inhibitor-transfected shRNA-Ascl2/LS174T cells (B–E). Error bars represent S.D. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.