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. 2014 Nov 4;289(52):36101–36115. doi: 10.1074/jbc.M114.598383

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Correlation between Ascl2 mRNA level and miR-200 family member levels in human colorectal carcinoma tissues. Quantitative real time PCR was performed in colon cancer tissues (CA) and their pericancerous mucosa (N) to quantitate Ascl2 mRNA levels and miR-200 family member levels. Ascl2 expression was higher in colon cancer samples (A), whereas miR-200b (B), miR-200a (C), miR-200c (E), and miR-141 (F) expression was lower in colon cancer samples; miR-429 (D) expression was at similar levels in colon cancer samples when compared with that in pericancerous mucosa. Ascl2 expression in colon cancer tissues was inversely correlated with miR-200b (G), miR-200a (H), miR-429 (I), miR-200c (J), or miR-141 (K) expression (*, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant).