TABLE 2.
The primary antibodies used in the experiment
WB, Western blotting; IHC, immunohistochemistry; ChIP, chromatin immunoprecipitation assay.
| Primary antibody | Protein molecular mass | Company | Dilution |
|---|---|---|---|
| kDa | |||
| Mouse monoclonal IgG to Ascl2 | 20 | Millipore (MAB4417) | WB, 1:1000 |
| Mouse polyclonal IgG to Ascl2 | 20 | Abcam (ab107046) | IHC, 1:100; WB, 1:500 |
| Mouse monoclonal IgG to Ascl2 | 20 | Millipore (MAB4418) | ChIP |
| Mouse monoclonal IgG to E-cadherin | 130 | Abcam (ab1416) | WB, 1:1000 |
| Rabbit polyclonal IgG to N-cadherin | 130 | Santa Cruz Biotechnology (sc7939) | WB, 1:500 |
| Rabbit polyclonal IgG to snail | 34 | Santa Cruz Biotechnology (sc28199) | WB, 1:500 |
| Rabbit polyclonal IgG to slug | 34 | Santa Cruz Biotechnology (sc15391) | WB, 1:500 |
| Rabbit polyclonal IgG to ZEB1 | 190 | Abcam (ab64098) | WB, 1:500 |
| Mouse monoclonal IgG to ZEB2 | 157 | Santa Cruz Biotechnology (sc271984) | WB, 1:500 |
| Mouse monoclonal antibody to actin | 42 | Beyotime Institute of Biotechnology (AA128) | WB, 1:1000 |