TABLE 4.

The primer sequences used in the microRNA quantitative real time PCR

F, forward primer; R, reverse primer.

Gene names	Primer pairs	Annealing temperatures	Product length
		°C	bp
U6	F: 5′-GCTTCGGCAGCACATATACTAAAAT-3′	60	89
	R: 5′-CGCTTCACGAATTTGCGTGTCAT-3′
hsa-miR-200a	F: 5′-GGGGTAACACTGTCTGGTAG-3′	60	63
	R: 5′-TGCGTGTCGTGGAGTC-3′
hsa-miR-200b	F: 5′-GGGGTAATACTGCCTGGT-3′	60	63
	R: 5′-TGCGTGTCGTGGAGTC-3′
hsa-miR-200c	F: 5′-GGTAATACTGCCGGGTAAT-3′	60	65
	R: 5′-CAGTGCGTGTCGTGGAGT-3′
hsa-miR-141	F: 5′-GGGGTAACACTGTCTGGTAA-3′	60	63
	R: 5′-TGCGTGTCGTGGAGTC-3′
hsa-miR-429	F: 5′-GGGGGTAATACTGTCTGGT-3′	60	64
	R: 5′-TGCGTGTCGTGGAGTC-3′