FIGURE 6.

Quantification of protein-thiol mixed disulfides in the lenses of WT and Grx2 KO mice. Two duplicate samples processed from six pooled lenses of the KO or WT mice were used for the protein mixed disulfide quantification. Details are described under “Experimental Procedures.” A, typical chromatograms of PSSC and PSSG analyses. PSSG and PSSC standards at 5 nmol (panel a) and 10 nmol (panel b); WT samples from 1 month (panel c), 7 months (panel e), and 16 months (panel g); KO samples from 1 month (panel d), 7 months (panel f), and 16 months (panel h). B, quantified PSSG level expressed as μmol/g wet lens weight. C, quantified PSSC level expressed as μmol/g wet lens weight. Data are expressed as means ± S.D. based on triplicate experiments. *, p < 0.05 versus age-matched WT; **, p < 0.01 versus age-matched WT.