FIGURE 2.

hAha1 prevents aggregation of denatured rhodanese. A, schematic view of yAha1 and hAha1 proteins used in the prevention of aggregation assay. The N-terminal sequences of yeast and human Aha1 are shown. B, Coomassie gel of purified yeast and human Aha1 proteins. C, prevention of rhodanese (Rho) aggregation. Denatured rhodanese was rapidly diluted to 0.5 μm into buffer alone or in buffer containing increasing amounts of GroEL at rhodanese:GroEL (14-mer) molar ratios of 1:0.125, 1:0.25, 1:0.5, and 1:1. D, Rhodanese was diluted into buffer alone or in buffer containing increasing amounts of BSA, (E) yAha1, (F) hAha1, (G) Δ22hAha1, (H) hAha1C, and (I) hAha1N as described under “Experimental Procedures.” Absorbance was recorded continuously every 2 s at 320 nm (A_{320 nm}) for 45 min. All curves are the means of three independent measurements.