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hAha1 prevents refolding of denatured luciferase. A, refolding of denatured luciferase in 1 μm Hsc70/2 μm Hsp40 is inhibited by increasing amounts of hAha1 but not affected by BSA. B, refolding of denatured luciferase in 20% RRL. hAha1 prevents refolding of denatured luciferase in contrast to BSA. Refolding is indicated as percentage of native luciferase. All measurements were performed in triplicate, and standard deviation is indicated by error bars.
