TABLE 2.
Oligonucleotides used in this study
| Name | Oligonucleotide sequence (5′ → 3′)a | Use |
|---|---|---|
| For expression of proteins | ||
| PRX301-F | CCATGGTGATCGCTCAAGGCCAAACT | Prx3 overexpression |
| PRX301-R | ACTAGTCGTTAGCTACCGATGAGCAAC | |
| GRX301-F | CACGGGACCATGGGCATGCCAAAGAT | GrxC overexpression |
| GRX301-R | CAATAAGTGTCGACTTAGTGACGGATTGC | |
| GR01-F | GAGAAAGCCATGGCAACGCATTTTGAC | GR overexpression |
| GR01-R | GTGCTTGTCGACTTAGCGCATCGTGACG | |
| For mutagenesis | ||
| PRX3C48S-F | GGCGTTCACGCCAACCAGCTCAGAAGCACATTTACCG | Construction of Prx3-C48S mutant |
| PRX3C48S-R | CGGTAAATGTGCTTCTGAGCTGGTTGGCGTGAACGCC | |
| PRX3C73S-F | GCGTCGATCTGATTGCCAGCGTTTCTGTCAACGATGC | Construction of Prx3-C73S mutant |
| PRX3C73S-R | GCATCGTTGACAGAAACGCTGGCAATCAGATCGACGC | |
| ISCR3CA1-F | CGCAACTAAGGCGCAGGGCAAAGGAGATGCGCAAGGCGGCAC | Construction of IscR3CA mutant |
| ISCR3CA1-R | GTGCCGCCTTGCGCATCTCCTTTGCCCTGCGCCTTAGTTGCG | |
| ISCR3CA2-F | CAAGGCGGCACTCGCGCGCTTACTCATACACTTTGGCGTGACC | |
| ISCR3CA2-R | GGTCACGCCAAAGTGTATGAGTAAGCGCGCGAGTGCCGCCTTG | |
| For mutant construction | ||
| PRX302-F | GTGGTTGATAGACCAGCCATTCGTC | Construction of prx3 mutant |
| PRX302-R | GATGGATCCTTTTTTGCCAGCAAA | |
| PRX303-F | AAAGGATCCATCGACAATGGCGTA | |
| PRX303-R | CGCTTTCGTGACCTTAATTGGTTTG | |
| For amplification of gene ORF | ||
| PRX304-F | CCATGGTGATCGCTCAAGGCCAAACT | Amplification of prx3 ORF |
| PRX304-R | GCATGCTTAGCTACCGATGAGCAACCA | |
| For primer extension analysis, EMSA, and DNase I protection assay | ||
| PRX305-F | ATATTTGGACATAAAAAGACCCCC | Amplification of prx3 upstream region |
| PRX305-R | CTAAAACAGGGTGGTGGACCA | Amplification of prx3 upstream region, Extension of prx3 transcript |
| For qRT-PCR | ||
| PRX3_qRT-F | TGAAAGCCTGGGGTGAAGCA | Expression of prx3 |
| PRX3_qRT-R | ATCGCGTAGCGTTGAGAGCG | |