FIGURE 5.

LC-MS analysis of leaf extracts of empty vector and CDS-expressing tobacco plants. A, negative mode LC-MS chromatograms of aqueous methanol extract of leaves of an empty vector tobacco plant (a) and a CDS-expressing tobacco plant (b). The most significantly different compound eluting at 45.93 min is indicated in the box, and it was only detected in CDS-expressing tobacco plants but not in empty vector control plants. B, the MS spectrum of this significantly different compound (a) and the inset (b) show the schematic of its collision-induced fragmentation. The compound was putatively identified as chrysanthemol conjugated to malonyl glucose.