S8A forward	5'-ggagcctgcacccgcccgaGctccgaggccccagc-3'
S8E forward	5'-ggagcctgcacccgcTcgaGAAccgaggccccagcag-3'
S8 reverse	5'-atggcgttgtcatcgtcatccttg-3'
T28;S36/38/40A forward	5'-atgcctccccccgagGcGccctctgaaggccgccagcccGCTcccG-CGccGGCccctacagagcgagc-3'
T28;S36/38/40E forward	5'-atgcctccccccgagGAAccctctgaaggccgGcagcccGAAccc-GAAcccGAAcctacagagcgagcc-3'
T28;S36/38/40 reverse	5'-ggtgggctcctggggccgggcggg-3'
S117A	5'-agtctgcagcggcgcTtAGcggtgtaccggcaga-3'
S117E forward	5'-tctgcagcggcgcctCGAggtgtaccggcagat-3'
S117E reverse	5'-ctctcgaaaaagacgttatccagggcggg-3'
S399/403;T409A forward	5'-aaGgaCgcGgcGgtGGCTaaAaaGgccGCcccagaggctgcca-gcGctcccagggacccta-3'
S399/403;T409E forward	5'-aaGgaCgcGgcGgtGGAGaaAaaGgccGAAccTgaggctgcc-agcGAAcccagggaccctatt-3'
S399/403;T409 reverse	5'-cccctgggtgatgcaagagctgag-3'
S480;T482;S493A forward	5'-cagaagaggaagtgcGCGcagGcccagtgccccaggaaggtcatca-agatggagGctgaggaggggaagg-3'
S480;T482;S493E forward	5'-cagaagaggaagtgcGAGcagGAAcagtgcccTaggaaggtcatc-aagatggagGAAgaggaggggaaggag-3'
S480;T482;S493	5'-ggctgtcgttgtattggagacatc-3'
S504/505A forward	5'-ggcaaggttggctcgAGCcGccccggagcagccca-3'
S504/505A reverse	5'-tccttcccctcctcagactccatc-3'
S504/505E forward	5'-gcaaggttggctcggGAAGAGccggagcagcccagg-3'
S505/505E reverse	5'-ctccttcccctcctcagactccat-3'
S518A forward	5'-cagcacctccaaggcagtcGcaccTccTcacctggatggaccg-3'
S518E forward	5'-cagcacctccaaggcCgtGGAaccaccccacctgga-3'
S518 reverse	5'-ggcctgggctgctccgg-3'
S527/530A	5'-ctggatggaccgcctGCccccaggGCccccgtcataggaag-3'
S527/530E forward	5'-ctggatggaccgcctGAAccTaggGAAcccgtcataggaagt-3'
S527/530E reverse	5'-gtggggtggtgagactgccttggag-3'
S560/561/562/565A forward	5′-cgcgttgtggtgatcGCGGCcGcggaagacGcagatgccgaaaact-3′
S560/561/562/565A reverse	5'-ttcctctgcctccccggcgccact-3'
S560/561/562/565E forward	5'-ggaggcagaggaacgTgttgtggtgatcGAAGAAGAggaagacGA-Agatgccgaaaactcg-3'
S560/561/562/565E reverse	5'-ccggcgccactggccacgtggttg-3'
S565A forward	5'-agcagctcggaagacGcagatgccgaaaact-3'
S565A reverse	5'-gatcaccacaacgcgttcctctgc-3'
S565E forward	5'-gttgtggtgatcagcTCTtcggaagacGAagatgccgaaaactc-3'
S565E reverse	5'-gcgttcctctgcctccccggcgcc-3'
V556/557/558A;I559S forward	5'-aggcagaggaacgcgCtgCggCTaGcagcagctcggaaga-3'
Δ476–490 forward	5'-gaggcaaggttggctcgga-3'
Δ476–490 reverse	5'-ctgggctgtcgttgtattggaga-3'
K65R	5'-atgccaggcggaagcGCGCtgcccgaagctgctg-3'
K160R forward	5'-acaccagtggttcctACGTcaTgaAgcccggcccctagca-3'
K160R reverse	5'-gcctcgaagcacttggcgcag-3'
K490R	5'-cccaggaaggtcatcCGgatggagtctgagga-3'
K616R forward	5'-gttttctttgacctcCGgattgacaatgaaa-3'
K616R reverse	5'-cagaggtctgtcttctgcttggg-3'