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. 2014 Nov 11;5(4):1032–1049. doi: 10.3390/genes5041032

Figure 1.

Figure 1

(a,b) Cartoon diagrams of illumination techniques of (a) wide-field and (b) narrow-field, showing volume of illuminated area. Note that in wide-field and narrow-field illumination, multiple nuclear pore complexes (NPCs) are illuminated; (c–g) Cartoon representations of data generated by various single-particle tracking (SPT) setups; (c) Typical 1D data showing path of an mRNA from the nucleus to the cytoplasm. The (green) center line is the experimentally determined position of the nuclear envelope (NE). The black (cytoplasm) and red (nuclear) lines are reference distances of 100 nm from the NE; (d) Histogram of the data in (c). Position of the molecule in 1D data is based on the position of the NE. Gaussian fitting could be interpreted as showing one (green) or two (red) areas of interaction of the molecule with the NPC; (e) An example of 2D data showing distribution of molecules (black dots) around the NPC (brown structure). N, nucleoplasm; C, cytoplasm; (f) Histogram of the distribution in (e) in the x dimension, showing fit of two Gaussian curves; (g) Histogram of the distribution in (e) in the y dimension, showing possible fit of multiple Gaussian curves. After Ma et al. [29] and Goryaynov et al. [9].