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. 2014 Nov 11;5(4):1032–1049. doi: 10.3390/genes5041032

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Diagrammatic representations of current major SPT setups. (a) Conventional fluorescence microscopy setup; (b) Parallax; (c) Biplane; (d) Angled mirror/side view; (e) Interference; (f) Astigmatism; (g) Double-helix point-spread function. S, sample; O, objective lens; L, lens; C, camera; M, mirror; BS, beam splitter; 3WBS, 3-way beam splitter; BPF, band-pass filter; CL, cylindrical lens; PF, phase filter. Distances between elements are not shown to scale and the positions of some elements are adjusted for clarity. After Deschout, et al. [91].