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. 2014 Dec 16;6(12):5028–5046. doi: 10.3390/v6125028

Figure 3.

Figure 3

Co-immunoprecipitation of CPB1 and E proteins from DENV2-infected and mock-infected Aedes aegypti primary midgut cell lysate. SDS-PAGE and western blot were performed using Dynabeads® conjugated with anti-CPB1 or anti-E antibodies. (A) 10% of the total cellular lysate. (B) Non-antibody-coated bead controls. Cell lysate derived from DENV2-infected Ae. aegypti primary midgut cells (infected). Control, cell lysate derived from mock-infected Ae. aegypti primary midgut cells. (C,D) Anti-CPB1 and anti-E antibodies pulled down the CPB1 and E protein from DENV2-infected samples (infected). For controls, anti-CPB1 antibody pulled down the CPB1 protein ((C), control), whereas no band was observed in the same sample when anti-E antibody was used ((D), control). Western blot analysis and β-actin protein levels of each sample are shown.