Figure 6.

Met inhibition suppresses anchorage-independent soft agar growth. (A) SW780 cells were treated with 5 nM of EMD1214063 (EMD063) for 20 min, followed by concurrent treatment with 1 nM HGF for an additional 20 min. Cell lysates were used for SDS-PAGE and immunoblot analysis of pMet, tMet, pAkt, tAkt, pErk and tErk; (B) Cell types indicated were embedded in soft agar, followed by 5 nM EMD1214063 (EMD063) treatment in the absence or the presence of HGF (1 nM) every other day. On day 7, MTT was used to quantitate viable cell colonies by absorbance at 570 nm. Values represent the mean ± SD of triplicate samples. Data are representative of at least three independent experiments; * indicates p < 0.05 vs. control.