Figure 7.
Cabozantinib inhibits SW780 xenograft tumor growth in SCID and hHGF/SCID mice. (A) SW780 cells were implanted subcutaneously on both flanks of SCID and hHGF/SCID mice (5 mice per group) and tumor growth over time was measured using calipers. Values represent the mean tumor volume ± SD, n = 10 tumors/group. Single asterisk indicates p < 0.05 vs. vehicle control group; (B,C) SW780 cells were implanted into both flanks of (B) SCID or (C) hHGF/SCID mice (5 mice per group). When tumor volume reached 150 mm3, mice were treated daily with either vehicle (circles) or cabozantinib (squares). Values represent mean tumor volume ± SD, n = 10 tumors/group, * indicates p < 0.05, ** indicates p < 0.001 vs. vehicle control; (D) Xenograft tumors from SCID (light gray bars) or hHGF/SCID (dark gray bars) mice from the time course in (A) through (C) were excised and processed by physical disruption and non-ionic detergent extraction; extracts were clarified by centrifugation and analyzed for total and pMet content. Bars represent the mean ± SD of triplicate samples from 5 tumors/group. Asterisks indicate significant differences at the p < 0.05 (*) and p < 0.001 (**) levels between bracketed groups.