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. 2014 Dec 8;6(12):5667–5678. doi: 10.3390/nu6125667

Figure 3.

Figure 3

Effects of Schisandrae Fructus ethanol extract on H2O2-induced reactive oxygen species (ROS) generation in C2C12 cells. C2C12 cells were pretreated with 500 μg/mL SFEE and/or 5 mM N-acetylcysteine (NAC) for 1 h and then stimulated with or without 1 mM H2O2 for 6 h. The cells were incubated at 37 °C in the dark for 20 min with new culture medium containing 10 μM 2′,7′-dichlorofluorescein diacetate (DCF-DA) to monitor ROS production. ROS generation was measured by flow cytometry. The results are mean ± SD values obtained from three independent experiments (* p < 0.05 compared with control group; # p < 0.05 compared with H2O2-treated group). SFEE, Schisandrae Fructus ethanol extract.