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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Mol Cell Endocrinol. 2014 Oct 27;398(0):89–100. doi: 10.1016/j.mce.2014.10.004

Figure 5.

Figure 5

Apoptosis in the seminiferous tubules of testis xenografts from mice treated with different doses of DBP. a – c) Fluorescence micrographs of a tissue section from a xenograft (0 mg/kg group) collected at 14 weeks in which TUNEL assay was performed. a) DAPI nuclear counterstaining, b) staining with anti-Digoxigenin-fluorescein antibody, c) merged image. Arrows in a) points to apoptotic structures (apoptotic nuclei or nuclear fragments) clearly visible in b) and c), bars = 50 μm. d) Percentage of tubules with presence of apoptotic structures. e) Number of apoptotic structures per seminiferous cord or tubule cross-section. In d) and e), different letters between bars of the same color indicate statistical difference (P<0.05).