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. 2015 Jan 6;12(102):20140797. doi: 10.1098/rsif.2014.0797

Figure 6.

Figure 6.

DUX4 perturbs Wnt/β-catenin signalling. (a) QPCR of downstream targets of Wnt/β-catenin signalling Lef1, Tcf3, Tcf4, Lgr5, Lgr6 and Myf5 in mouse satellite cell-derived myoblasts, 24 or 48 h after infection with either DUX4 or control retroviral constructs. Data are mean ± s.e.m. from three mice, where an asterisk denotes a significant difference (p < 0.05) from infection with control retrovirus using a paired two-tailed Student's t-test. (b) TopFLASH/FopFLASH assay of immortalized C2C12 murine myoblasts transfected with either DUX4 or control plasmid, shows a significant increase in TopFLASH/FopFLASH luciferase activity with DUX4 expression (n = 4). Data are mean ± s.e.m. from four independent experiments where an asterisk denotes significance (p < 0.05) from control using a two-tailed Student's t-test.