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. 2014 Dec 17;9:127–146. doi: 10.2147/DDDT.S68501

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© 2015 He et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Effect of SCE, SchA, and SchB on Nrf2 protein stability in human hepatocellular liver carcinoma cell line cells.

Notes: Human hepatocellular liver carcinoma cell line cells were treated with the protein synthesis inhibitor CHX at 1 µg/mL for 10, 20, 30, or 60 minutes with or without pretreatment of 200 µg/mL SCE, 20 µM SchA, or 200 µM SchB for 4 hours, and then the Nrf2 protein level was determined by Western blotting assay. (A) Representative blots of Nrf2 from human hepatocellular liver carcinoma cell line cells. (B) The Nrf2 protein levels over 60 minutes when cells were treated with CHX±SCE, SchA, or SchB. Data are presented as the mean ± standard deviation (n=3) and the half-lives were analyzed by one-way analysis of variance.

Abbreviations: SCE, Schisandra chinensis extract; Sch, schisandrin; Nrf2, nuclear factor (erythroid-derived 2)-like 2; CHX, cycloheximide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; min, minutes.