Table 1.
Sequences of primers designed for real-time polymerase chain reaction analysis
| Gene | Forward primer | Reverse primer |
|---|---|---|
| Nrf2 | ATAGCTGAGCCCAGTATC | CATGCACGTGAGTGCTCT |
| HO-1 | ACATCTATGTGGCCCTGGAG | TGTTGGGGAAGGTGAAGAAG |
| NQO1 | CAGTGGTTTGGAGTCCCTGCC | TCCCCGTGGATCCCTTGCAG |
| GCLM | AATCTTGCCTCCTGCTGTGTGA | TGCGCTTGAATGTCAGGAATGC |
| GSTA4 | AGTTGCAGGATGGTAACCACC | ATGGCCTAAAGATGTTGTAGACGG |
| MRP2 | CTTCGGAAATCCAAGATCCTGG | TAGAATTTTGTGCTGTTCACATTCT |
| MRP4 | GGATCCAAGAACTGATGAGTTAAT | TCACAGTGCTGTCTCGAAAATAG |
| P-gp | TGCTCAGACAGGATGTGAGTTG | AATTACAGCAAGCCTGGAACC |
| OATP1A2 | AAGACCAACGCAGGATCCAT | GAGTTTCACCCATTCCACGTACA |
| OATP1B1 | TGAAATCACTTGCACTGGGTTT | CTCCTAGTGCGTATAACCATTGAGT |
| GAPDH | AGAAGGCTGGGGCTCATTTG | AGGGGCCATCCACAGTCTTC |
Abbreviations: Nrf2, nuclear factor (erythroid-derived 2)-like 2; HO-1, heme oxygenase-1; NQO1, NAD(P)H:quinone oxidoreductase 1; GCLM, glutamate–cysteine ligase, modifier subunit; GSTA4, glutathione S-transferase A4; MRP, multidrug resistance-associated protein; P-gp, P-glycoprotein; OATP, organic anion transporting polypeptide; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.