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Determination of the optimal ratio of TycA to TycB1 for d-Phe-Pro-DKP formation. Both enzymes were incubated in the presence of l-phenylalanine, l-proline, ATP, and magnesium chloride, and the reactions were quenched after 1 h by immediate organic extraction. DKP was prepared as described in Materials and Methods and analyzed by HPLC. All samples were taken and analyzed in triplicate.
