Table 3. Identified Differentially Accumulating Phenolics in Inner- and Outer-Stem Tissues of the lbf1 Mutant as Revealed by LC-MS.
| Outer Tissues | Inner Tissues | ||||
|---|---|---|---|---|---|
| tR (min) | Compound | Wild Type | lbf1 | Wild Type | lbf1 |
| 7.75 | Coniferin | n.d. | n.d. | n.d. | 131,603 ± 28,678 |
| 10.48 | Syringin | n.d. | n.d. | n.d. | 4,788 ± 695 |
| 12.88 | *G(8-O-4)G' hex | n.d. | n.d. | n.d. | 4,294 ± 2,419 |
| 13.30 | *G(8-O-4)G' hex | n.d. | n.d. | n.d. | 6,187 ± 3,826 |
| 14.90 | *G(8-O-4)G' hex | n.d. | n.d. | 401 ± 201 | 11,431 ± 3,315 |
| 15.12 | G(e8-O-4)S hex | n.d. | n.d. | n.d. | 25,94 ± 866 |
| 15.79 | G(8-5)G hex | n.d. | n.d. | 9,062 ± 4,375 | 266,534 ± 46,161 |
| 15.86 | G(t8-O-4)G | 1,466 ± 1,078 | n.d. | 13,168 ± 2,265 | 19,750 ± 10,882 |
| 15.87 | Lariciresinol hex | n.d. | n.d. | n.d. | 5,803 ± 1,963 |
| 16.36 | G(e8-O-4)G | 1,738 ± 1,060 | n.d. | 14,266 ± 2,556 | 23,642 ± 9,543 |
| 16.70 | *G(e8-O-4)FA | 50,087 ± 22,282 | 7,725 ± 3,722 | 4,327 ± 1,503 | 11,461 ± 5,925 |
| 21.48 | Lariciresinol | 3,533 ± 980 | n.d. | 2,808 ± 1,866 | 6,582 ± 2,210 |
| 21.57 | G(t8-O-4)secoisolariciresinol | 2,153 ± 441 | n.d. | 342 ± 73 | 985 ± 369 |
| 22.17 | G(e8-O-4)lariciresinol | 3,208 ± 32 | n.d. | 8,056 ± 2,475 | 5,673 ± 1,576 |
| 22.79 | *G(t8-O-4)lariciresinol | 764 ± 83 | n.d. | 1,429 ± 465 | 925 ± 263 |
| 23.65 | *G(8-5)FA | 2,474 ± 327 | n.d. | 129 ± 53 | 1,089 ± 362 |
| 24.54 | G(t8-O-4)S(8-5)G | 873 ± 237 | n.d. | 29,468 ± 5,586 | 28,725 ± 8,515 |
| 24.93 | G(t8-O-4)Sred/S(8-8/5)Gred/G | 1,377 ± 614 | n.d. | 2,184 ± 522 | 1,322 ± 373 |
| 25.80 | G(e?8-O-4)G(8-5)G' | 338 ± 3 | n.d. | 9,551 ± 1,938 | 4,253 ± 1,477 |
| 27.55 | *G(t8-O-4)S(8-8)S | 1,554 ± 457 | n.d. | 2,457 ± 802 | 4,462 ± 1,308 |
| 28.86 | *G(e8-O-4)S(8-8)S | 232 ± 52 | n.d. | 593 ± 144 | 963 ± 272 |
Values represent the relative abundance based on the extracted ion chromatogram, expressed as per mg dry weight tissue; values significantly different (Student’s t test) from the wild type at P < 0.05 are indicated in bold or italics, respectively, when they are higher or lower in abundance. n.d., not detected. Nomenclature is based on Morreel et al. (2004). Guaiacyl units, syringyl units, and units derived from ferulic acid and coniferaldehyde are referred to as G, S, FA, and G’, respectively. The linkage type is indicated in parentheses. “red,” reduced unit or adjacent linkage (Morreel et al., 2010a). A forward slash indicates that two units or two linkage positions are equally possible at this position in the shorthand name. hex, hexose or hexoside; tR, retention time. Asterisks indicate compounds that have not been described previously in flax stem tissue. Spectral data and structures of these compounds are given in Supplemental Figure 2. The spectral data of the other compounds are described by Huis et al. (2012). Three wild-type and six lbf1 plants were analyzed. Values are means ± se. Only the differentially accumulating metabolites with known identities are shown.