Figure 3.

ATL1-Induced Cell Death Is Dependent on the RING Domain and Is Suppressed by Coexpression with EDR1.

(A) and (B) Overexpression of ATL1 induces cell death in N. benthamiana, which is dependent on a functional RING domain. The ATL1-mCherry constructs were expressed under control of a 35S promoter, while the EDR1-sYFP constructs were under control of a dexamethasone-inducible promoter. Dexamethasone was applied 16 to 18 h after infiltration and photographs were taken 48 h after infiltration. (B) shows ion leakage data. Error bars represent sd; n = 3. This experiment was repeated four times with similar results.

(C) and (D) ATL1-mCherry-induced cell death in N. benthamiana is suppressed by coexpression with EDR1-sYFP, but not with the substrate trap mutant version, stEDR1-sYFP. (D) shows ion leakage data. Infiltration of Agrobacterium carrying an empty 35S promoter vector was used as the negative control and is shown in (D) only.