Os BZR1 Can Directly Bind to the Promoters of GA20ox-2, GA3ox-2, GA2ox-3, and D2.
(A) Distribution of BZR1 binding cis-elements in the 2-kb region preceding the initial codon (−1) of GA20ox-2, GA3ox-2, GA2ox-3, and D2. Gray and black arrows indicate that BZR1 binding could presumably promote and repress gene expression, respectively, according to a previous report.
(B) Enrichment fold of Os BZR1 binding sites compared with the samples without antibody application (NoAb) by qPCR analysis. The amplified regions are indicated in (A). All values were normalized to an unrelated ACTIN1 intron region, and segments located in the GA2ox-3 3′ untranslated region (UTR) and DLT coding region were used as negative controls.