Figure 3.

Constitutive Expression of RDO5 During Seed Maturation Enhances Seed Dormancy.

(A) Immunoblot analysis of RDO5:TAP protein accumulation in seeds of three independent RDO5:TAP overexpression transgenic lines.

(B) Germination after different periods of dry storage of RDO5:TAP overexpression transgenic lines and wild-type Ler. Shown are averages ± se of six to eight independent batches of seeds for each genotype.

(C) Immunoblot analysis of RDO5:HA protein accumulation of transgenic rdo5-2 lines, containing RDO5 driven by the heat shock promoter (ProHS). Protein was isolated from siliques after they had received 3 h of heat induction (37°C) during three subsequent days that started at 10, 13, 16, or 19 d after pollination. The top panel shows representative siliques of the four stages.

(D) Immunoblot analysis of RDO5:HA protein in freshly harvested seeds from siliques that had been heat treated at different stages after pollination as indicated in (C). The germination percentage (averages ± se) of seeds from six independent batches for each genotype is shown at the bottom.

(E) Immunoblot analysis of RDO5:HA protein in after-ripened seeds after 6 h of heat induction that was given at 3, 6, 12, and 24 h after imbibition. The germination percentage (averages ± se) of seeds from six independent batches for each genotype is shown at the bottom.

[See online article for color version of this figure.]