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(A) Irs2 and Pck1, involved in the regulation of lipid metabolism, were up-regulated by RNA-Seq analysis and confirmed by qRT-PCR. (B) and (C) confirm the genes with the greatest up-regulation and down-regulation, respectively. The direction of the change was confirmed for all 6 genes, though not necessarily to the same degree. ** - p-value <0.005, *** - p-value <0.0001.
