Figure 2. CAR protein levels after PB treatment.

Fifteen microgram aliquots of liver nuclear extract or whole lysate were used for western blot analysis as described in materials and method section. Nuclear accumulation of CAR after 6 h PB treatment in WT and KO males on the left. On the right, total CAR protein levels in liver whole lysate prepared from PB-treated WT and KO males. We used TBP and β-Actin as loading control of nuclear extract and whole lysate, respectively. Columns denote mean ± SE determined in at 3 individual animals. Opened and closed columns represent control and PB-treated animals, respectively. Unpaired t-test was used to compare the relative CAR levels between genotypes (*WT vs KO, p<0.05). This experiment was repeated twice with different sample set prepared from different animals.