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. 2014 Dec 26;9(12):e115663. doi: 10.1371/journal.pone.0115663

Figure 4. The recruitment of transcription factors and histone de-methylation after PB treatment.

Figure 4

(A) The mRNA expressions of CYP2B10 in the livers of WT, KO and DKO mice after 3 h PB treatment. Columns denote mean ± SD determined in triplicates. Opened and closed columns represent control and PB-treated animals, respectively. (B) PB-induced recruitment of CAR/RXRα and RNA polymerase II in the Cyp2b10 promoter in the livers of WT and KO; WT and DKO mouse liver. Precipitation of DNA fragments by anti-RXRα and anti-RNA polymerase II antibodies at PBREM and TATA box-containing region, respectively, was determined. (C) Histone de-methylation after PB treatment in the Cyp2b10 promoter of WT and KO; WT and DKO mouse liver. Precipitation of DNA fragments by antibodies against the repressive mark H3K27me3. For both, precipitation of DNA fragments by normal IgG was used as negative control and DNA fragments without immunoprecipitation (Input) were used as positive control. Data shown are representative of results from two individual experiments.