Table 3.

Estimates of mutational variance for fitness assays

									ΔV per		CVm ancestor5 (% per		CVm MA6 (% per
	Variance1 (95% CIs)				F-ratio test2		ΔV3		generation4 = Vm		generation)		generation)
Strain	First Anc	First MA	Second Anc	Second MA	First	Second	First	Second	First	Second	First	Second	First	Second
CC-1952	0 (0,0)	6.93×10−4 (3.56×10−4, 1.89×10−3)	6.99×10−5 (3.19×10−5, 2.57×10−4)	5.99×10−4 (3.15×10−4, 1.55×10−3)	NA*	8.57*	6.93×10−4	5.29×10−4	6.79×10−7	5.18×10−7	0.54	0.55	0.82	0.66
CC-2342	2.86×10−17 (1.30×10−17, 1.05×10−16)	1.47×10−3 (6.42×10−4, 6.08×10−3)	2.43×10−4 (1.11×10−4, 8.90×10−4)	9.82×10−4 (5.16×10−4, 2.55×10−3)	5.14×1013*	4.04*	1.47×10−3	7.39×10−4	1.42×10−6	7.16×10−7	0.78	0.65	1.06	0.85
CC-2344	0 (0,0)	6.3×10−4 (3.31×10−4, 1.64×10−3)	0 (0,0)	1.09×10−4 (5.75×10−5, 2.84×10−4)	NA*	NA*	6.30×10−4	1.09×10−4	6.72×10−7	1.16×10−7	0.77	0.30	0.89	0.32
CC-2931	5.24×10−17 (2.39×10−17, 1.93×10−16)	1.061×10−4 (5.46×10−5, 2.89×10−4)	1.99×10−4 (9.07×10−5, 7.30×10−4)	7.76×10−4 (4.16×10−4, 1.93×10−3)	2.02×1012*	3.9*	1.06×10−4	5.77×10−4	1.03×10−7	5.63×10−7	0.19	0.58	0.25	0.71
CC-2937	−	−	7.15×10−21 (3.26×10−21, 2.62×10−20)	2.91×10−4 (1.56×10−4, 7.24×10−4)	−	4.07×1016*	−	2.91×10−4	−	3.13×10−7	−	0.51	−	0.54

Columns show estimates from the first and second replicate assays. Anc = ancestor; MA = mutation accumulation lines.

¹Among MA line variance component.

²Ancestral among-pseudoline variance component divided by the between line MA variance component; the critical value = 3.006 with df = 15,9 (^*significant difference at the 5% level between MA and ancestral variance).

³Change in the among-line variance component of fitness between the MA lines and their ancestors.

⁴Mutational heritability calculated as ΔV /number of generations.

⁵Coefficients of mutational variance calculated as the square root of ΔV per generation divided by the mean fitness of the ancestral lines.

⁶Coefficients of mutational variance calculated as the square root of ΔV per generation divided by the mean fitness of the MA lines.