Table 1. The kinds of reagents.
| * | Reagent | Target | Clone | Conjugate | Company |
| Flow, IF | Anti-rat RT1Aa,b Ab | MHC class I | C3 | FITC | BioLegend, San Diego, CA |
| IF | Anti-rat CD3 Ab | T cell | 1F4 | FITC | BioLegend, San Diego, CA |
| IF | Anti-rat CD4 Ab | CD4+ T cell | 10B5 | Purified | Abcam, Tokyo, Japan |
| Flow | Anti-rat CD4 Ab | CD4+ T cell | W3/25 | APC-Cy7 | BioLegend, San Diego, CA |
| Flow | Anti-rat CD6 Ab | T cell | OX-52 | FITC | BD Pharmingen, San Diego, CA |
| Flow, IF | Anti-rat CD8α Ab | CD8+ T cell | G28 | PE | BioLegend, San Diego, CA |
| IF | Anti-rat CD45 Ab | White blood cell | OX-1 | PE-Cy7 | BioLegend, San Diego, CA |
| IHC | Anti-human CD3 Ab | T cell | Rabbit, Polyclonal | Purified | DAKO, Glostrup, Denmark |
| IHC | Anti-rat CD8α Ab | CD8+ T cell | OX-8 | Purified | BioLegend, San Diego, CA |
| IHC | Anti-rat CD68 Ab | Macrophage | ED1 | Purified | BMA BIOMEDICALS, Augst, Switzerland |
| Flow | Anti-rat Granulocyte Marker Ab | Monocyte, Macrophage | HIS48 | Biotin | eBioscience, San Diego, CA |
| Flow | Streptavidin | PE-Cy7 | BioLegend, San Diego, CA | ||
| Flow, IF | Anti-mouse CD16/32 Ab | Fcγ receptor III/II | 2.4G2 | Culture sup | ATCC**, Manassas, VA |
| IF | Anti-mouse IgG1 Ab | IgG1 | Goat, Polyclonal | TRITC*** | SouthernBiotech, Birmingham, AL |
| IF | Anti-rat IgM Ab | IgM | Goat, Polyclonal | FITC | MP Biomedicals, Tokyo, Japan |
| IF | Anti-rat IgG Ab | IgG | Rabbit, Polyclonal | FITC | MBL, Nagoya, Japan |
| IF | Anti-rat C3 Ab | C3 | Goat, Polyclonal | FITC | MP Biomedicals, Tokyo, Japan |
| IF | Anti-rat RT1B Ab | MHC class II | OX-6 | PE | BioLegend, San Diego, CA |
*Assessment: Flow: Flow cytometory; IHC: Immunohistochemistry; IF: Immunofluorescence.
**American Type Culture Collection.
***Tetramethylrhodamine isothiocyanate.