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. 2014 Dec 26;9(12):e115841. doi: 10.1371/journal.pone.0115841

Figure 1. Hsp90 expression in human sperm under various conditions.

Figure 1

Sperm were stained with Hsp90 antibody (red) or rabbit IgG (control). Hoechst 33258 staining (blue) marks cell nuclei. A) Negative control, rabbit IgG used as the primary antibody. B) Sperm not subjected to Percoll isolation. C) Sperm isolated using Percoll isolation (0 h) D) Sperm isolated using Percoll under capacitation conditions (3 h). Bar  = 10 µm. Experiments were performed using specimens collected from five different men.