Skip to main content
. 2014 Dec 26;9(12):e115841. doi: 10.1371/journal.pone.0115841

Figure 2. Effect of Geld on Hsp90 expression during capacitation.

Figure 2

A) Under capacitation conditions, sperm were incubated in absence of geldanamycin (Geld) as a blank control for 0 h (lane 1) and 3 h (lane 2); with dimethyl sulfoxide (DMSO) as the vehicle control (lane 3), 1 µM Geld (lane 4), and 20 µM Geld (lane 5) for 3 h. Protein concentrations of sperm lysates were quantified using the Bicinchoninic Acid Assay Kit. Equal amounts of protein were resolved by SDS-PAGE, transferred to Immunoblot-P membrane, and probed with Hsp90 antibody. B) Band intensity ratios of Hsp90 to β-tubulin were lower in the Geld-treated sperm compared with that treated with the DMSO vehicle control, *P<0.05 (four independent experiments using ten different semen samples). Band intensities were measured using Image J software.