FIG. 7.

Demonstration of intracellular replication of Salmonella serovar Dublin within A. rhysodes. The percentage of viable ampicillin-resistant bacteria was determined for the inoculum and for bacteria recovered 2 and 16 h after challenge, and segregation of the ampicillin resistance plasmid was used as a readout for the efficiency of replication. (A) Replication of invasive and noninvasive Salmonella serovar Dublin and Salmonella serovar Typhimurium. (B) Replication of the hilA, spvR, and spvB mutants and of the plasmid-cured strain. wt, wild type; plasmid-, plasmid cured. Values are means from three independent experiments, each carried out in duplicate wells.