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. 2014 Dec 26;9(12):e115944. doi: 10.1371/journal.pone.0115944

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© 2014 Jacquet et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Ten mice were immunized with H1-HFBI as indicated in the Experimental procedures. Anti-HA antibodies were assayed by ELISA in the pre-immune sera (open circle) and the sera collected after the 4^th (blue triangle) and 6^th (red square) boost. Plates were coated with 5 µg/ml of recombinant Influenza A/Texas/05/2009(H1N1) ectodomain expressed in mammalian cells (Sino Biologicals, 11085-V08H). HRP-conjugated anti-mouse secondary antibody was used for detection. HA titer was calculated as the highest dilution giving a signal higher than three times the signal coming from the negative control. (b) Box and whisker analysis of antibody titers obtained after endpoint ELISA titer analysis of the test groups. Each dot represents the antibody titer from an individual mouse. (p-values = 0.18 (boost 4/boost 6), 1.8.10⁻⁴ (boost 4/pre-immune), 3.8.10⁻⁴ (boost 6/pre-immune))