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. 2014 Dec 26;9(12):e115854. doi: 10.1371/journal.pone.0115854

Figure 5. Effect of GSK3 inhibitor CHIR99021 on IL-1β-induced pro-inflammatory cytokine IL-6 in adipose tissue and skeletal muscle.

Figure 5

Human (A,B) omental adipose tissue and (C,D) skeletal muscle were incubated with 1 ng/ml IL-1β in the absence or presence of 10 µM CHIR99021 (CHIR) for 20 h (n = 6 patients). (A,C) Gene expression for IL-6 was analysed by qRT-PCR. Gene expression was normalised to GAPDH mRNA expression and the fold change was calculated relative to IL-1β. Data displayed as mean ±SEM. *P<0.05 vs. IL-1β (one-way ANOVA). (B,D) The incubation medium was assayed for concentration of IL-6 release by ELISA. Data displayed as mean ±SEM. *P<0.05 vs. IL-1β (one-way ANOVA).