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. 2014 Dec 26;9(12):e115854. doi: 10.1371/journal.pone.0115854

Figure 10. Effect of GSK3 inhibitor SB216763 on LPS-induced pro-inflammatory cytokines and chemokines in adipose tissue.

Figure 10

Human omental adipose tissue was incubated with 10 µg/ml LPS in the absence or presence of 20 µM SB216763 (SB) for 20 h (n = 5 patients). (A–E) Gene expression for TNF-α, IL-1β, IL-6, IL-8 and MCP-1 was analysed by qRT-PCR. Gene expression was normalised to GAPDH mRNA expression and the fold change was calculated relative to LPS. Data displayed as mean ±SEM. *P<0.05 vs. LPS (one-way ANOVA). (F–J) The incubation medium was assayed for concentration of TNF-α, IL-1β, IL-6, IL-8 and MCP-1 release by ELISA. Data displayed as mean ±SEM. *P<0.05 vs. LPS (one-way ANOVA).