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. 2014 Dec 26;9(12):e115949. doi: 10.1371/journal.pone.0115949

Figure 6. The effects induced by heat intervention were partially attenuated by silencing β-catenin in HCCLM3 cells.

Figure 6

(A) Immunofluorescence staining indicated that β-catenin expression was partially attenuated by silencing of CTNNB1 in HCCLM3 cells. (B) The protein levels of β-catenin measured by western blot analysis after siCTNNB1 transfection. (C) Representative images of migration and invasion of HCCLM3-mock cells and HCCLM3-siCTNNB1 cells in transwell assay. HCCLM3-mock cells and HCCLM3-siCTNNB1 cells were cultured 48 h after 45°C heat intervention and normal condition, respectively, RT-PCR (D) showed the relative mRNA expression of transcription factors Snail, Slug, and Twist. Western blot (E) revealed the expression of EMT related transcription factors and Cyclin-D1.