Skip to main content
. 2014 Dec 26;9(12):e116062. doi: 10.1371/journal.pone.0116062

Figure 2. CARMA proteins associates with endogenous DEPDC7.

Figure 2

A) Total cell lysates (30 µg) prepared from the indicated cell lines were separated by SDS-PAGE and transferred onto membranes subsequently probed with the affinity purified anti-DEPDC7 antisera. B-D) HEK-293 cells were transiently cotransfected with FLAG-tagged versions of the indicated constructs. 24 hrs later, cell lysates were immunoprecipitated with anti-DEPDC7 (B, C) or with anti-CARMA3 (D) antisera and analyzed for coprecipitating proteins by immunoblot assay.