Table 3. Oligonucleotide primers and TaqMan* fluorescent probes used in this study.
Gene | Primer | Primer Sequences | Amplicon size (bp) | References |
blaOXA51-like | OXA51like-F | 5'-AACATTAAAGCACTCTTACTTATAAC | 171 | [22] |
OXA51like-R | 5′-TTGTTGGATAACTAAAACACCCGT | |||
OXA51like-Dye | FAM-CTCACCTTATATAGTGTCTGCTAA-BHQ1 | |||
blaOXA23-like | OXA23-F1 | 5′-TGCTCTAAGCCGCGCAAATA | 130 | [39] |
OXA23-R1 | 5′-TGACCTTTTCTCGCCCTTCC | |||
OXA23-probe | FAM-GCCCTGATCGGATTGGAGAACCA-BHQ1 | |||
blaOXA24-like | OXA24-F | 5′-CAAATGAGATTTTCAAATGGGATGG | 123 | [39] |
OXA24-R | 5′-TCCGTCTTGCAAGCTCTTGAT | |||
OXA24-probe | FAM-GGTGAGGCAATGGCATTGTCAGCA-BHQ1 | |||
blaOXA58-like | OXA58-F | 5′-CGCAGAGGGGAGAATCGTCT | 102 | [39] |
OXA58-R | 5′-TTGCCCATCTGCCTTTTCAA | |||
OXA58-probe | FAM-GGGGAATGGCTGTAGACCCGC- BHQ1 | |||
blaOXA143-like | OXA-143-F | 5′-TGGCACTTTCAGCAGTTCCT | 149 | [40] |
OXA-143-R | 5′-TAATCTTGAGGGGGCCAACC | |||
blaNDM | NDM-F | 5′-GGTGCATGCCCGGTGAAATC | 661 | [41] |
NDM-R | 5′-ATGCTGGCCTTGGGGAACG | |||
ISAba1 | ISAba1 | 5′-CATTGGCATTAAACTGAGGAGAAA | 451 | [42] |
ISAba2 | 5′-TTGGAAATGGGGAAAACGAA | |||
blaOXA51-like | OXA-69A | 5′-CTAATAATTGATCTACTCAAG | 975 | [12] |
OXA-69B | 5′-CCAGTGGATGGATGGATAGATTATC | |||
rpoB | Ac696F | 5′-TAYCGYAAAGAYTTGAAAGAAG | 350 | [23] |
Ac1093R | 5′-CMACACCYTTGTTMCCRTGA |
*Eurofins MWG Operon, Courtaboeuf, France.