Table 1. Detection of PIK3CA mutation (E542K, E545K, H1047R) by DNA sequencing and digital PCR analysis (only among the patients whose mutations were detected).
| Patient ID | Mutation | |
| Direct sequencing Digital PCR analysis (mutant percentage) | ||
| 31 | H1047R, E542K | H1047R (58.2%), E542K (41.0%) |
| 10 | H1047R | H1047R (28.9%) |
| 40 | H1047R | H1047R (25.8%), E542K (0.3%), E545K (0.2%) |
| 18 | H1047R | H1047R (19.2%) |
| 37 | H1047R | H1047R (16.7%), E542K (0.1%) |
| 15 | H1047R | H1047R (6.8%) |
| 41 | H1047R | E542K (0.2%) |
| 27 | E542K | E542K (24.6%) |
| 8 | E545K | E545K (20.9%) |
| 23 | Wild-type | H1047R (7.7%) |
| 34 | Wild-type | H1047R (5.1%), E545K (0.9%) |
| 3 | Wild-type | H1047R (1.5%) |
| 16 | Wild-type | H1047R (0.8%) |
| 36 | Wild-type | H1047R (0.7%) |
| 1 | Wild-type | H1047R (0.6%) |
| 7 | Wild-type | H1047R (0.6%) |
| 12 | Wild-type | H1047R (0.5%), E545K (0.3%) |
| 26 | Wild-type | H1047R (0.4%), E542K (0.1%) |
| 13 | Wild-type | E542K (0.4%) |
| 39 | Wild-type | E545K (0.3%) |
| 28 | Wild-type | E542K (0.1%), E545K (0.1%) |
| Frequencya | 21% | 49% |
a
Frequency of the patient with mutated DNA.